![]() Activation of the reporter gene provides a quantifiable measurement of the degree of interaction between the target receptor and the protease-tagged arrestin partner. The blue to green fluorescence ratio obtained directly correlates to receptor modulation by agonists or antagonists. There, the transcription factor directly regulates transcription of a beta-lactamase reporter construct, which is measured upon addition of the live-cell substrate (Figure 2). This transcription factor immediately enters the nucleus, thus bypassing additional signaling intermediates. ![]() The protease-tagged arrestin cleaves a protease site fused to the C-terminus of the GPCR, releasing a non-native transcription factor. The Tango GPCR Assay System (Figure 1) is a proprietary platform based upon ligand binding to a specific G protein-coupled receptor (GPCR) that triggers desensitization, a process mediated by the recruitment of protease-tagged arrestin proteins to the activated receptor. ![]() Request Assay Information Use Assay Maker CFR Tsunami Tsunami foi um ride adquirido pelo Playcenter em 2011, e permaneceu no parque at o dia de seu fechamento.
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